Saturday, January 23, 2016

Is it Possible that DNA Genetic Material itself to fool the Definite Determination? Part IV

Police detectives shall be smart enough to out-smart the Gangsters’ smart thinking for Gang- Raping and Murder


Collection of samples for DNA investigations
In very first of my series articles I discussed about sperm swimming to reach the ovulated ova at the top of uterus or further down – animal to animal this may vary – but the fertilized egg have to be implanted within the uterus(normally) for further development ensuring a next generation for that species. This is nature’s code survival of each species. 
Single sperm
The sperm of the all animals, particularly of mammals well adapted to swim and reach the ovum and as such sperm heads are perfect DNA preservation capsules protecting the nuclear DNA against the all environmental hazards of desiccation, such as by acid, alcohol, laboratory materials etc.  Thus semen material, found at the crime scene of rape, however contaminated it can be, should be an absolutely perfect material for pinpointing the identified or arrested suspects, - though all depends on the efficiency of the laboratory handles the job.  In this modern era if a laboratory sends negative reply for a judicial query on the basis of semen sample as of desiccated or contaminated, and that particular laboratory has no any value in judiciary and the state cannot sway from the responsibility of establishing or finding alternatives to fit into the modern day universal requirements regarding law enforcement against the heinous crimes.
It is truly laborious for any laboratory to type the semen samples that are even somewhat “fresh” from the victim who was gang raped and murdered. In such cases court shall give ample time to the laboratory (requested by the lab) if they have accepted the compounded mixed personal semen samples to pinpoint the each and every culprit.  
In this respect, the law enforcement personals have to act smart and efficiently with commitment to outsmart the assumption of the gang that the gang raping would confuse even the laboratory for final determination and supply and support the lab with additional crime scene collection, such as hair, empty bottles and cans of beverages, bottle lids that could have been mouthed by the suspects, smeared clothes, any objects that could have abraded the suspects, in a hurry to finish a desire, the crime for a thrill.  In one of the completely blind folded cases –in Germany a triple murder was solved through a wrist watch found at the scene many days after the crime had taken place. In many developed countries like Germany it is the police that do the entire lab work – in this particular case as soon they found the wristwatch they gave a simple wash that ensured collections of some cell debris – they were able to collect some epithelial cells of the skin from the grooves of wrist wind of the watch. In this particular case Bundeskriminalamt (BKA)—the German Federal Bureau of Investigation would have depended on a technique presently popular among the western nations including USA known as “GENETIC FINGER PRINTINGwhich is not available in Sri Lanka and also not (yet) ready for such a sophisticated system. But German Federal Bureau of Investigation is totally against this Genetic Finger Printing for lot bureaucratic involved.  

But what I emphasize here is sample resources for complete evidence that go with the collections of crime scene.   Sri Lanka is depended on RFLP analysis and PCR reaction test (which would be explained later), both of which are very effective as well if the lab work is so efficient to extract DNA needed—around 10 µg at least.   The same amount need for Genetic Finger Printing, as well which is nothing to do with the normal finger printing but rather with the Nucleus of the entire cells of the body, but like in fingerprinting  genetic information of the criminals are stored for future references.     
It should be noteworthy that any Colour reactions and staining that are used in criminal investigations, mostly to obtain fingerprint patterns (from real fingers) from a crime scene,  Surprisingly, neither superglue vapours nor any of these stains—amido black, leucomalachite green, Hungarian Red or luminol—interfere with DNA typing off the epithelial cells of entire skin. 

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